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Table 2 Experimental data sources for concentrations and related literature in PD. The values for modeling the concentrations of individual genes involved in the biochemical pathways of PD were adapted from experimental data listed as references

From: Computational modelling of TNFα related pathways regulated by neuroinflammation, oxidative stress and insulin resistance in neurodegeneration

Protein/gene name

Experiment

Region

Model

Concentration

References

Control

Diseased

TNF α

Sandwich enzyme immunoassay (EIA)

CSF

Humans (in vitro)

22.3 + 9.5 pg/ml

96.3 + 9.1 pg/ml

(Mogi et al. 1994)

Glutamate

Ion exchange chromatography

Blood plasma

Humans (in vitro)

34.1 + 11.3 μmol/L

71.7 + 8.5 μmol/L

(Iwasaki et al. 1992)

Ca 2+

Fluorometric analysis

Substantia nigra pars compacta

Sprague-Dawley rats. (in vitro)

0.080 μM

100 μM

(Putney and Tomita 2011)

TNFR1

ELISA

Blood serum

Humans (in vitro)

438.9 ± 171.9 pg/ml

558.5 ± 246.3 pg/ml

(de JR de Paula et al. 2009)

αS

IMR assay

Blood plasma

Humans (in vitro)

645.57 pg/ml

1294.9 pg/ml

(Oczkowska et al. 2014)

ROS

Assay for aconitase activity. Parallel assay of α-KGDH activity and H2O2 generation by α-KGDH Statistics.

Brain cortex

Guinea Pig (in vitro)

12.7 pmol/min/mg

32 pmol/min/mg

(Tretter and Dam-Vizi 2004)

IL-1β

ELISA

CSF

Human (in vitro)

9.409 pg/mL

11.122 pg/mL

(Hu et al. 2015)

Tau

Sandwich ELISA

CSF

Human (in vitro)

640 ± 230 pg/ml

720 ± 590 pg/ml

(Blennow et al. 1995)