Table 1 The PDB structure IDs of Lipase A and its six mutants used for analysis, with the mutated sites and their optimum functional temperature
From: Complex network analysis of thermostable mutants of Bacillus subtilis Lipase A
PDB ID | Mutations | Optimum Temperature( ̊C) |
|---|---|---|
1I6W (WT) | WILD-TYPE | 35 |
1T4M (M1) | N166Y, A132D | -- |
1T2N (M2) | N166Y, A132D, L114P | 45 |
3D2A (M3) | N166Y, A132D, L114P, I157M | -- |
3D2B (M4) | N166Y, A132D, L114P, I157M, F17S, N89Y | 50 |
3D2C (M5) | N166Y, A132D, L114P, I157M, F17S, N89Y, A15S, A20E, G111D | 55 |
3QMM (M6) | N166Y, A132D, L114P, I157M, F17S, N89Y, A15S, A20E, G111D, M134E, M137P, S163P | 65 |