Fig. 3
From: Mapping functional connectivity of bursting neuronal networks

Simultaneous laser scanning photostimulation (LSPS) and calcium (Ca) imaging. a Bright-field and image showing positions of photostimulated neuron (circle) and responding neurons (triangle and square). Scale bar: 50 μm. b Ca responses (fractional change in Fluo-4 fluorescence) 2 s after photostimulation. Scale bar: 50 μm. c Ca responses of corresponding neurons as a function of time. Scale bars: 5% (vertical) and 5 s (horizontal)